tag:blogger.com,1999:blog-36840063.post3955461038429676644..comments2024-03-27T22:57:00.742+00:00Comments on Hyperlipid: Dairy and diabetesPeterhttp://www.blogger.com/profile/14527788116058656094noreply@blogger.comBlogger44125tag:blogger.com,1999:blog-36840063.post-89134019664310141262022-07-28T04:40:32.813+00:002022-07-28T04:40:32.813+00:00Hi Peter,
I'd love to see your review of thi...Hi Peter, <br /><br />I'd love to see your review of this study: http://robbwolf.com/wp-content/uploads/2010/02/European-Journal-of-Clinical-Nutrition-Milk.pdf<br /><br />This study showed that milk intake raised fasting insulin levels and insulin resistance. It was a very short study (only 7 days) so I don't know how much stock can be put in it but the result is still curious. The other issue is that the milk was skimmed! Still, it'd be great to see one of your technical takes on this that explains the result here.<br /><br />I wonder whether the intake of whole milk would give the same result...I have my doubts.Antoniohttps://www.blogger.com/profile/13932953251955983142noreply@blogger.comtag:blogger.com,1999:blog-36840063.post-39285390286933207832020-02-01T18:43:45.509+00:002020-02-01T18:43:45.509+00:00I've changed my daily walk to add in some hill...I've changed my daily walk to add in some hillclimbing which serves very well to get my heart rate up. As my fitness improves, I can find steeper hills to climb—plenty of them around here! I prefer to avoid the jarring involved in running. I already have to work to fend off plantar fasciitis in one foot (avoiding pavement helps a lot).cavenewthttps://www.blogger.com/profile/08461541719892430585noreply@blogger.comtag:blogger.com,1999:blog-36840063.post-61617830845441767532020-02-01T18:11:53.570+00:002020-02-01T18:11:53.570+00:00Certainly is all about the mitochondria. And any a...Certainly is all about the mitochondria. And any anything good seems to be triggered by fatty acids without hyperglycaemia/hyperinsulinaemia...<br /><br />BTW I've always been very anti-running. More than a little embarrassing to admit to pro-running colleagues at work that I now do this!<br /><br />PeterPeterhttps://www.blogger.com/profile/14527788116058656094noreply@blogger.comtag:blogger.com,1999:blog-36840063.post-73230276355130607242020-02-01T17:59:20.542+00:002020-02-01T17:59:20.542+00:00Thanks, Peter! I've never before wanted a heal...Thanks, Peter! I've never before wanted a health tracker, but I just received a heart rate monitor so I can implement this. Very encouraging that you think it's on the right track. <br /><br />Years ago in my forum I posted commentary titled "It's All About The Mitochondria". That was before I knew any of this metabolic stuff—at the time I was just reading about autoimmunity and nerve cells. Looking back, it seems mildly prescient.cavenewthttps://www.blogger.com/profile/08461541719892430585noreply@blogger.comtag:blogger.com,1999:blog-36840063.post-2847477250842738462020-02-01T06:22:35.015+00:002020-02-01T06:22:35.015+00:00Hi cave,
I picked up MAF training via Grant Schof...Hi cave,<br /><br />I picked up MAF training via Grant Schofield (co-worker with George) on twitter, I've been using it since October last year. It's very hard to exercise down to the MAF HR when you are over 60! I'm using the upper limit he allows (127/min) for "fit" elderly having started using 117/min. My impression is that he is correct on HR training. I've not allowed myself any anaerobic running yet. I'll try that in March. There are subtleties of my weight changes which suggest his concept of fasting mimicking exercise on insulin levels is spot on. That might be useful to yourself. Training while actually fasting allows you to go a little quicker without having to drop back to a brisk walk all the time! I'm still bouldering in between times.<br /><br />PeterPeterhttps://www.blogger.com/profile/14527788116058656094noreply@blogger.comtag:blogger.com,1999:blog-36840063.post-15110951131943826792020-01-30T16:22:31.172+00:002020-01-30T16:22:31.172+00:00Hap—
My understanding is that fasting (to promote...Hap—<br /><br />My understanding is that fasting (to promote autophagy) and exercise promote mitogenesis and repair. I had always understood that it required strenuous aerobic exercise, but recently I read about the MAF technique of exercise and I am interested in that. George Henderson is a contributor to this site which lends it credibility in my eyes. <br /><br />https://profgrant.com/2019/07/19/why-fitness-is-medicine-and-you-should-learn-about-fasting-mimicking-exercise-fme/<br /><br />Despite reading for years Peter and others about mitochondria, I recently ran across the first mention ever (for me) of mitochondrial fusion/fission. I'm curious about how important this might be in the context of Protons.cavenewthttps://www.blogger.com/profile/08461541719892430585noreply@blogger.comtag:blogger.com,1999:blog-36840063.post-13156916707469465892020-01-29T17:17:44.209+00:002020-01-29T17:17:44.209+00:00wondering if there are stimuli to repair/replace m...wondering if there are stimuli to repair/replace mitochondria with varying degrees of impairment, even if pre diabetic or fulminant T2D? ROS from RET might signal apoptosis? right kind of exercise...biogenesis? Eliminate environmental/nutritional toxins (N6) to spare the newbies? Most likely any new mitochondria will have some of the damaged/defective genetic material but maybe not so much.Haphttps://www.blogger.com/profile/03013326676353754009noreply@blogger.comtag:blogger.com,1999:blog-36840063.post-86411457494810671302019-11-07T05:00:51.550+00:002019-11-07T05:00:51.550+00:00cave, treatment-wise "the cognoscenti always...cave, treatment-wise "the cognoscenti always do better". The average medical professional demonstrably knows less than yourself. That's you off to the best start you can get...<br /><br />All the best<br /><br />PeterPeterhttps://www.blogger.com/profile/14527788116058656094noreply@blogger.comtag:blogger.com,1999:blog-36840063.post-80695636486724435852019-11-06T23:21:18.584+00:002019-11-06T23:21:18.584+00:00JR, interesting history.
Somewhat off-topic, one ...JR, interesting history.<br /><br />Somewhat off-topic, one sentence quoted from Gofman jumped out at me:<br /><br />'“In patients who said they did not adhere to a low fat, low cholesterol diet, the recurrence in death rate was four times as high as in [heart attack] patients who stated that they adhered to the diet” (p. 325).'<br /><br />Wow. Fat and cholesterol are so evil that they cause four times as many people to *die twice*.cavenewthttps://www.blogger.com/profile/08461541719892430585noreply@blogger.comtag:blogger.com,1999:blog-36840063.post-4421251252623828522019-11-06T22:55:22.839+00:002019-11-06T22:55:22.839+00:00Happy to be reminded of this old post. It reinforc...Happy to be reminded of this old post. It reinforces my self-prescribed adjuvant dietary regime for cancer, to minimize glucose (Warburg) and insulin (growth promoter), and boost ketones. I'm avoiding chicken/eggs and pork (PUFA) and eating a dose of cacao nibs daily (stearate).<br /><br />So far the only cancer center medical professional I've been able to engage on the subject – my oncologist's nurse – insists a low-fat diet is the best. Sigh.cavenewthttps://www.blogger.com/profile/08461541719892430585noreply@blogger.comtag:blogger.com,1999:blog-36840063.post-59270958893890917762019-11-06T13:19:35.327+00:002019-11-06T13:19:35.327+00:00Prof. Noakes makes a very nice reconstruction of t...Prof. Noakes makes a very nice reconstruction of times and motives of "conception"...<br />https://www.crossfit.com/essentials/its-the-insulin-resistance-stupid-part-6<br />JRJRhttps://www.blogger.com/profile/15237161564054970075noreply@blogger.comtag:blogger.com,1999:blog-36840063.post-7623239838891056062016-10-10T16:53:25.817+00:002016-10-10T16:53:25.817+00:00Not diabetes, but involves dairy, did you see this...Not diabetes, but involves dairy, did you see this one? https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4809873/Ally Houstonhttps://www.blogger.com/profile/02357253446763432043noreply@blogger.comtag:blogger.com,1999:blog-36840063.post-80834582038734481862016-05-18T14:56:18.394+00:002016-05-18T14:56:18.394+00:00I just thought the dairy-insulin thing was interes...I just thought the dairy-insulin thing was interesting in the context of this post, as many people report trouble with sustaining weight loss when consuming full dairy on a keto diet. So, while the high sat. fat content might be generally beneficial, milk/cheese/yogurt might not be wholly beneficial in themselves on a HF-LC diet. So I think its important to distinguish between full dairy vs things like heavy cream/sour cream/double cream/cream cheese. <br /><br />The podcast itself is interesting. He talks about taking 1g metformin/day, and pretty much all blood-markers improving except for testosterone, which dropped on metformin and returned to normal levels once he stopped taking it. He also talks a lot about exogenous ketone use, and studies he has done on rats using exogenous ketones to prevent seizures, etc. I'm sure all of this is old news. Kinda surprising that someone seemingly at the forefront of keto research would maintain an archaic view on LDL. Rattushttps://www.blogger.com/profile/03181576895593679474noreply@blogger.comtag:blogger.com,1999:blog-36840063.post-31040946890625419562016-05-17T20:52:07.091+00:002016-05-17T20:52:07.091+00:00Yes, people have their individual reactions to var...Yes, people have their individual reactions to various foods. But I'm interested in the time point when the lipid hypothesis converted from complete bollocks, i.e. the 1950s, to something believable. I must have blinked!<br /><br />PeterPeterhttps://www.blogger.com/profile/14527788116058656094noreply@blogger.comtag:blogger.com,1999:blog-36840063.post-83581678352822664122016-05-17T17:35:04.578+00:002016-05-17T17:35:04.578+00:00"Heavy cream, sour cream and butter in large ..."Heavy cream, sour cream and butter in large amount increased my LDL (and LDL-p). Simply replacing the heavy cream with coconut cream and cutting my sour cream consumption in half (to 2 servings/day) caused the LDL to go back down."<br /><br />I have 0 knowledge about the implications of having high LDL, but he viewed it as a bad sign. I'm assuming that you view his concern as unfounded, and that this is another example of dogma vs newer insights. <br /><br />Also, since we are talking about dairy, I think my problems stemmed from whole dairy products [milk, cheese w. high lactose] raising insulin, and fermented dairy containing histamine. Sour cream = can't breathe. Normal cream = no probs. Rattushttps://www.blogger.com/profile/03181576895593679474noreply@blogger.comtag:blogger.com,1999:blog-36840063.post-17772853798172862812016-05-17T16:03:21.277+00:002016-05-17T16:03:21.277+00:00And lowering LDLc is good???????
PeterAnd lowering LDLc is good???????<br /><br />PeterPeterhttps://www.blogger.com/profile/14527788116058656094noreply@blogger.comtag:blogger.com,1999:blog-36840063.post-38875773548296535772016-05-17T15:34:48.897+00:002016-05-17T15:34:48.897+00:00Tim Ferriss podcast with Dom D'Agostino. Dom t...Tim Ferriss podcast with Dom D'Agostino. Dom talked about how his LDL went quite high when he ate 500,1000 surplus dairy calories a day. When he cut the dairy [sour cream/cream] intake in half and used coconut cream instead, his LDL went back down. Thoughts?Rattushttps://www.blogger.com/profile/03181576895593679474noreply@blogger.comtag:blogger.com,1999:blog-36840063.post-70038588999594278802016-05-01T14:33:44.958+00:002016-05-01T14:33:44.958+00:00@Passthecream,
Right, I'm trying to find the ...@Passthecream,<br /><br />Right, I'm trying to find the simplest & robust 'substrate => product' assay done in control & cancer cells in both normoxic & hypoxic conditions, with a 1-substrate change at a time (the 1 variable isolation shtick already discussed in the comments of previous posts).<br /><br />I've started reading Nick Lane's "Vital Question" (better late than never, right??).<br /><br />If yo want to suggest anything more please do by email, I'm raphi.inter[at]gmail.com<br /><br />Cheers!raphihttps://www.blogger.com/profile/08992252569979714724noreply@blogger.comtag:blogger.com,1999:blog-36840063.post-34156167677842788742016-05-01T02:19:20.131+00:002016-05-01T02:19:20.131+00:00Ps monochromatic light (defocused lasers, leds) an...Ps monochromatic light (defocused lasers, leds) and absorbance spectra constitute another interesting and cheap bunch of techniques for probing cells.Passthecreamhttps://www.blogger.com/profile/01214860448492630477noreply@blogger.comtag:blogger.com,1999:blog-36840063.post-37207965655900566952016-05-01T02:12:16.449+00:002016-05-01T02:12:16.449+00:00Raphi, metabolism isn't my area unfortunately ...Raphi, metabolism isn't my area unfortunately just a badly educated enthusiasm, but if i were to hazzard a guess I'd suggest the response of a cell culture under very tightly controlled conditions to forced steps in the various substrates taken one at a time, measuring the anticipated outputs. Straightaway that tells you that I'd prefer it if there was some sort of biological oscilloscope and signal generator available. I dont know if step-functions per se are explicitly used in biology much, probably implicitly only, but at the edge there is all sorts of interesting information. Oh and you'd compare the response of your culture to a supposedly healthy one.<br /><br />I recall in the last chapter or tho of Powrer sex suicide etc, Nick Lane mentions a technique he developed to test mitochondrial health of organs pre-transplant. Might be worth a look.<br /><br /><br />I cant remember if any of those links I pasted mentioned citrate but that's another interesting line to follow in this area as an inhibitor of phosphofructokinase. <br /><br /><br />C.Passthecreamhttps://www.blogger.com/profile/01214860448492630477noreply@blogger.comtag:blogger.com,1999:blog-36840063.post-30624571025183265822016-04-30T15:07:01.004+00:002016-04-30T15:07:01.004+00:00@passthecream,
all great links. they contain many...@passthecream,<br /><br />all great links. they contain many avenues i want to go down but cant, given the constraints of the 'simple' in vitro experiment im designing.<br /><br />You seem to take 'old school' (aka proper) science to heart, so i'll ask you:<br /><br />what is the simplest way to convince you cancer cells are or aren't using their mitochondria normally (aka respiring)?raphihttps://www.blogger.com/profile/08992252569979714724noreply@blogger.comtag:blogger.com,1999:blog-36840063.post-45509868039368740022016-04-30T14:49:13.431+00:002016-04-30T14:49:13.431+00:00@Passthecream,
Thanks, I'm having a look at t...@Passthecream,<br /><br />Thanks, I'm having a look at them now.raphihttps://www.blogger.com/profile/08992252569979714724noreply@blogger.comtag:blogger.com,1999:blog-36840063.post-86497022548482219792016-04-30T14:46:38.425+00:002016-04-30T14:46:38.425+00:00Raphi, not exactly what you're looking for but...Raphi, not exactly what you're looking for but a few links to papers in that general area that I've stumbled over lately: <br /><br />http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4581199/<br /><br />http://www.ncbi.nlm.nih.gov/pubmed/22350013<br /><br />http://www.nature.com/nature/journal/v452/n7184/pdf/nature06734.pdf<br /><br />http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2714668/<br />Passthecreamhttps://www.blogger.com/profile/01214860448492630477noreply@blogger.comtag:blogger.com,1999:blog-36840063.post-55315420584948604472016-04-25T20:21:19.836+00:002016-04-25T20:21:19.836+00:00A bit of thinking to do there raphi!
PeterA bit of thinking to do there raphi!<br /><br />PeterPeterhttps://www.blogger.com/profile/14527788116058656094noreply@blogger.comtag:blogger.com,1999:blog-36840063.post-24030241424373713192016-04-25T13:04:11.928+00:002016-04-25T13:04:11.928+00:00@Peter,
Gotcha, quantity - the insulin response m...@Peter,<br /><br />Gotcha, quantity - the insulin response might thus have more to do with the AA bolus, as dairy products are calorically dense.<br /><br />The AA input into TCA is of particular interest to me currently as im designing my MSc thesis experiment on supposedly oxidative cancer cell lines...Im trying to parse how much of of mitochondrial contribution to total ATP turnover is from true OxPhos vs mitochondrial fermentation. There are big energy gaps to account for in the research as well as the 'missing oxygen' piece.<br /><br />So there's what you mentioned 1) Asparagine & Aspartate entering @ oxaloacetate.....then there's also 2) Phenylalanine & Tyrosine entering @ Fumarate..... then 3) Isoleucine, Methionine, Threonine & Valine entering @ Succinyl-CoA.... & lastly 4) Glutamate => (derived from Glutamine, Arginine, Histidine or Proline) entering @ alpha-ketoglutarate.<br /><br />One premise of the Mitochondrial Theory of cancer is that mitochondria are functioning differently...so differently that some might describe them as broken: the inputs aren't turned into ATP because of 1) uncoupling (& proton leak?) and 2) amino acid fermentation rather than OxPhos.<br /><br />Yet when rotenone & other mitochondrial inhibitors are used to lower oxygen use, the cells produce very little ATP compared to before. The authors then conclude "yep, cancer cells have perfectly fine, respiring mitochondria".<br /><br />I'm not so confident given that mitochondrial inhibitors hurt multiple complexes which could affect other non-respiring activities going on...what says you? What do you think I should look at to figure out the yes-respiring / no-respiring question?raphihttps://www.blogger.com/profile/08992252569979714724noreply@blogger.com